PUBLICATIONS
Shang WH, Hori T, Martins NM, Toyoda A, Misu S, Monma N, Hiratani I, Maeshima K, Ikeo K, Fujiyama A, Kimura H, Earnshaw WC, Fukagawa T.
Chromosome engineering allows the efficient isolation of vertebrate neocentromeres.
Developmental Cell 24:635-648 (2013)
ニワトリのセントロメアの複製タイミングがS期後期と親和性が高いことが示唆された（＝国立遺伝研・深川研究室との共同研究）。
[PubMed] [Developmental Cell] [ReplicationDomain]
Pope BD, Tsumagari K, Battaglia D, Ryba T, Hiratani I, Ehrlich M, Gilbert DM.
DNA replication timing is maintained genome-wide in primary human myoblasts independent of D4Z4 contraction in FSH muscular dystrophy.
PLoS One 6:e27413 (2011)
[PubMed] [PLoS One] [ReplicationDomain] [PDF]
Ryba T, Hiratani I, Sasaki T, Battaglia D, Kulik M, Zhang J, Dalton S, Gilbert DM.
Replication timing: a fingerprint for cell identity and pluripotency.
PLoS Computational Biology 7:e1002225 (2011)
[PubMed] [PLoS Computational Biology] [ReplicationDomain] [PDF]
Ryba T, Battaglia D, Pope BD, Hiratani I, Gilbert DM.
Genome-scale analysis of replication timing: from bench to bioinformatics.
Nature Protocols 6:870-895 (2011)
我々が確立したDNA複製タイミングの全ゲノムマッピング法のプロトコール一式。
[PubMed] [Nature Protocols] [PMC version PDF]
Hiratani I, Gilbert DM.
Autosomal lyonization of replication domains during early mammalian development. In: The Cell Biology of Stem Cells. Landes Bioscience and Springer (edited by Eran Meshorer and Kathrin Plath).
Advances in Experimental Medicine and Biology 695:41-58 (2010) [Review]
1960年の発見から半世紀間のDNA複製タイミング研究の流れ、その中で我々が果たした役割、そして将来の方向性を論じた総説論文。帰国前に書き上げ、帰国後の研究の指針となっている作品。我々の研究に興味を持たれた方にはまずこの論文を読むことをお薦めします。
[PubMed] [Advances in Experimental Medicine and Biology] [PDF]
Gilbert DM, Takebayashi SI, Ryba T, Lu J, Pope BD, Wilson KA, Hiratani I.
Space and time in the nucleus: developmental control of replication timing and chromosome architecture.
Cold Spring Harbor Symposia on Quantitative Biology 75:143-153 (2010) [Review]
[PubMed] [Cold Spring Harbor Symposia on Quantitative Biology]
Ryba T, Hiratani I, Lu J, Itoh M, Kulik M, Zhang J, Schulz TC, Robins AJ, Dalton S, Gilbert DM.
Evolutionarily conserved replication timing profiles predict long-range chromatin interactions and distinguish closely related cell types.
Genome Research 20:761-770 (2010)
ヒトとマウスの複製タイミング及びその発生制御様式の高い進化的保存性に加えて、複製タイミングとHi-C（＝近年注目を集める染色体高次構造の指標の１つ）の驚くほど高い相関を見出した。複製タイミングが、細胞種特異的な染色体高次構造の有力なゲノムワイド数的指標であることを改めて実証した論文。
[PubMed] [Genome Research] [ReplicationDomain] [PDF]
Pope BD, Hiratani I, Gilbert DM.
Domain-wide regulation of DNA replication timing during mammalian development.
Chromosome Research 18:127-136 (2010) [Review]
[PubMed] [Chromosome Research] [PMC version PDF]
Hiratani I, Ryba T, Itoh M, Rathjen J, Kulik M, Papp B, Fussner E, Bazett-Jones DP, Plath K, Dalton S, Rathjen PD, Gilbert DM.
Genome-wide dynamics of replication timing revealed by in vitro models of mouse embryogenesis.
Genome Research 20:155-169 (2010)
ES細胞分化の系を中心にマウス胚初期発生のモデル細胞22種類を解析。DNA複製タイミングマップが細胞種特異的な染色体高次構造の良い数的指標であることを細胞生物学的解析によって実証し、その上で染色体高次構造が大きく変化する分岐点が三胚葉分化直前のエピブラストの時期に存在することを提唱した論文。
[PubMed] [Genome Research] [ReplicationDomain] [PDF]
Yokochi T, Poduch K, Ryba T, Lu J, Hiratani I, Tachibana M, Shinkai Y, Gilbert DM.
G9a selectively represses a class of late-replicating genes at the nuclear periphery.
Proceedings of the National Academy of Sciences USA 106:19363-19368 (2009)
ヒストン修飾の一つH3K9me2はLate S複製とよく相関するが、この修飾酵素G9aを欠失させても複製タイミングの発生制御に影響は出なかった。しかし核膜周辺の遺伝子群が特異的に再活性化されており、G9aの欠失が核膜周辺のヘテロクロマチン環境を変化させた可能性が示唆された。
[PubMed] [Proceedings of the National Academy of Sciences USA] [ReplicationDomain] [PDF]
Hiratani I, Takebayashi S, Lu J, Gilbert DM.
Replication timing and transcriptional control: beyond cause and effect. Part II.
Current Opinion in Genetics & Development 19:142-149 (2009) [Review]
DNA複製タイミング制御の研究から何が見えてくるのか。2002年のPart I [Gilbert DM (2002) Curr Opin Cell Biol 14:377-383]に引き続き、その意味する所を思索した総説論文。
[PubMed] [Current Opinion in Genetics & Development] [PMC version PDF]
Hiratani I, Gilbert DM.
Replication timing as an epigenetic mark.
Epigenetics 4:93-97 (2009) [Review]
[PubMed] [Epigenetics] [PDF]
Weddington N*, Stuy A*, Hiratani I*, Ryba T, Yokochi T, Gilbert DM. (*Equal Contributions)
ReplicationDomain: a visualization tool and comparative database for genome-wide replication timing data.
BMC Bioinformatics 9:530 (2008)
DNA複製タイミングマップのデータベースサイトReplicationDomainの立ち上げとその紹介。
[PubMed] [BMC Bioinformatics] [ReplicationDomain] [PDF]
Hiratani I, Ryba T, Itoh M, Yokochi T, Schwaiger M, Chang CW, Lyou Y, Townes TM, Schübeler D, Gilbert DM.
Global reorganization of replication domains during embryonic stem cell differentiation.
PLoS Biology 6:e245 (2008)
DNA複製タイミングの高解像度全ゲノムマッピング法を哺乳類細胞において初めて確立し、マウスES細胞分化に伴う複製タイミング発生制御がゲノムの広範囲に渡ることを示した論文。予想を越えたダイナミックな染色体高次構造の発生制御が示唆され、その後の一連の研究成果につながった。
[PubMed] [PLoS Biology] [Faculty of 1000] [ReplicationDomain] [PDF]
Hiratani I, Leskovar A, Gilbert DM.
Differentiation-induced replication-timing changes are restricted to AT-rich/long interspersed nuclear element (LINE)-rich isochores.
Proceedings of the National Academy of Sciences USA 101:16861-16866 (2004)
[PubMed] [Proceedings of the National Academy of Sciences USA] [PDF]
Hiratani I, Yamamoto N, Mochizuki T, Ohmori SY, Taira M.
Selective degradation of excess Ldb1 by Rnf12/RLIM confers proper Ldb1 expression levels and Xlim-1/Ldb1 stoichiometry in Xenopus organizer functions.
Development 130:4161-4175 (2003)
[PubMed] [Development] [PDF]
Hikasa H, Shibata M, Hiratani I, Taira M.
The Xenopus receptor tyrosine kinase Xror2 modulates morphogenetic movements of the axial mesoderm and neuroectoderm via Wnt signaling.
Development 129:5227-5239 (2002)
[PubMed] [Development] [PDF]
Hiratani I, Mochizuki T, Tochimoto N, Taira M.
Functional domains of the LIM homeodomain protein Xlim-1 involved in negative regulation, transactivation, and axis formation in Xenopus embryos.
Developmental Biology 229:456-467 (2001)
[PubMed] [Developmental Biology] [PDF]
Tsuji T, Sato A, Hiratani I, Taira M, Saigo K, Kojima T.
Requirements of Lim1, a Drosophila LIM-homeobox gene, for normal leg and antennal development.
Development 127:4315-4323 (2000)
[PubMed] [Development] [PDF]
日本語総説
平谷伊智朗
DNA複製タイミングの発生過程における制御
細胞工学 27:1013-1019 (2008)
[細胞工学] [PDF]