| Speaker: |
Azusa Inoue
Harvard Medical School |
| Title: |
Epigenetic reprogramming of mouse sperm genome following fertilization |
| Summary : | Terminally differentiated sperm is epigenetically reprogrammed to a totipotent state after fertilization by maternal factors. During this process, dynamic epigenetic events, including de novo nucleosome assembly and global DNA demethylation, occur in the paternal genome of mouse zygotes. In my talk, I would like to introduce our recent discoveries including:
1). The mechanism of the DNA demethylation of paternal genome: we have revealed that Tet3-mediated conversion of 5-methylcytosine into 5-hydroxymethyl-, 5-formyl-, and 5-carboxyl-cytosine followed by DNA replication-dependent dilution accounts for paternal DNA demethylation.
2). The biological role of de novo nucleosome assembly: we successfully generated a nucleosome-depleted paternal genome by inhibition of maternal histone incorporation in zygote. Analyses of the nucleosome-free paternal pronucleus revealed that de novo nucleosome assembly is required for nuclear pore complex assembly. |
