Labs

in vitro Histogenesis

Team Leader
Mototsugu Eiraku (Ph.D.)

In vitro generation of a functional organ with complex structures is one major goal for the field of developmental and cell biology. To achieve this goal, it is a reasonable strategy to recapitulate the ontogeny, which is the most efficient and robust process for organogenesis that has been acquired through evolution, in an in vitro system. Our laboratory aims to clarify the molecular and cellular dynamics underlying organogenesis, and to develop new technologies for in vitro recapitulation, that is, three-dimensional functional organ formation from stem cells. These researches aim to make important contributions to the field of developmental biology, stem cell biology, and regenerative medicine.

News List
2016.2.9
Hippocampal neurons from human ES cells
2015.4.21
Induction of retinal tissue with ciliary margin from hESCs
2011.4.7
The self-made eye: Formation of optic cup from ES cells
2008.11.6
Cerebral cortical tissue from ES cells
Select References

Hasegawa Y, et al. Emergence of dorsal-ventral polarity in ESC-derived retinal tissue. Development 143 (21), 3895-3906 (2016). doi: 10.1242/dev.134601

Sakaguchi H, et al. Generation of functional hippocampal neurons from self-organizing human embryonic stem cell-derived dorsomedial telencephalic tissue. Nat Commun 6, 8896 (2015). doi:10.1038/ncomms9896

Kuwahara A, et al. Generation of a ciliary margin-like stem cell niche from self-organizing human retinal tissue. Nat Commun 6, 6286 (2015). doi:10.1038/ncomms7286

Okuda S, et al. Vertex dynamics simulations of viscosity-dependent deformation during tissue morphogenesis. Biomech Model Mechanobiol 14, 413–425 (2015). doi:10.1007/s10237-014-0613-5

Kadoshima T, et al. Self-organization of axial polarity, inside-out layer pattern, and species-specific progenitor dynamics in human ES cell-derived neocortex. Proc Natl Acad Sci U S A 110, 20284–20289 (2013). doi:10.1073/pnas.1315710110

Nakano T, et al. Self-formation of optic cup and storable stratified neural retina from human ESCs. Cell Stem Cell 10, 771–785 (2012). doi:10.1016/j.stem.2012.05.009

eiraku[at]cdb.riken.jp

Recruit

Self-organized optic cup formation from ES cells
ES cell-derived neuroepithelium (lifeact-GFP)
Photoreceptor cell (red) and bipolar cells (green) in ES cell-derived neural retina
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