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| Animal Resources and Genetic Engineering (Shinichi Aizawa) |
| On April 1, 2007, the Laboratory for Animal Resources and Genetic Engineering was reorganized to include the following two units |
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Shinichi Aizawa, Ph.D.;
Unit Leader |
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Genetic Engineering Unit (Shinichi Aizawa)
The Genetic Engineering Unit works with research labs within the CDB, as well as other labs in Japan and throughout the Asia-Pacific region to develop mutant mice useful to the study of development and regeneration. In these joint development projects, we receive sequence information for genes of interest from our collaborators, and perform all subsequent stages of the development from construction of the targeting vector to generation of chimeras, making about 100 new knockout mutants every year. We also develop transgenic mice for the CDB and Kansai biological research communities. In addition, we develop new bioimaging technologies to aid in the visualization of mutant mouse embryos at the tissue, cell and organelle level. |
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Kazuki Nakao, Ph.D.;
Unit Leader |
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Animal Resource Unit
(Kazuki Nakao)
The Animal Resource Unit maintains and cares for CDB's laboratory mouse and rat resources in a specific pathogen free (SPF) environment, as well as handling the shipping and receiving of mutant mice both within the CDB and with other domestic and international institutions. In addition, the Unit provides support services for the IVF breeding of animals, including cleaning, colony development, and cryopreservation by vitrification. We also provide pregnant females, mouse embryos, and surrogates to support the CDB animal experimentation community, and develop technologies for the study of rodent reproductive biology. |
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Shioi G, et al. A mouse reporter line to conditionally mark nuclei and cell membranes for in vivo live-imaging. Genesis 49.570-8 (2011)
Yoshikawa Y, et al. The M-Ras-RA-GEF-2-Rap1 pathway mediates tumor necrosis factor-alpha dependent regulation of integrin activation in splenocytes. Mol Biol Cell 18. 2949-59 (2007)
Takashima Y, et al. Neuroepithelial cells supply an initial transient wave of MSC differentiation. Cell 129. 1377-88 (2007)
Takasaki N, et al. Acetylated YY1 regulates Otx2 expression in anterior neuroectoderm at two cis-sites 90 kb apart. Embo J 26. 1649-59 (2007)
Ohtani N, et al. Visualizing the dynamics of p21(Waf1/Cip1) cyclin-dependent kinase inhibitor expression in living animals. Proc Natl Acad Sci U S A 104. 15034-9 (2007)
Niwa Y, et al. The initiation and propagation of Hes7 oscillation are cooperatively regulated by Fgf and notch signaling in the somite segmentation clock. Dev Cell 13. 298-304 (2007)
Nakao H, et al. Metabotropic glutamate receptor subtype-1 is essential for motor coordination in the adult cerebellum. Neurosci Res 57. 538-43 (2007)
Kurotaki Y, et al. Blastocyst axis is specified independently of early cell lineage but aligns with the ZP shape. Science 316. 719-23 (2007)
Fujishima K, et al. Targeted disruption of Sept3, a heteromeric assembly partner of Sept5 and Sept7 in axons, has no effect on developing CNS neurons. J Neurochem 102. 77-92 (2007)
Chan T, et al. Ripply2 is essential for precise somite formation during mouse early development. FEBS Lett 581. 2691-6 (2007)
Oda M, et al. DNA methylation regulates long-range gene silencing of an X-linked homeobox gene cluster in a lineage-specific manner. Genes Dev 20. 3382-94 (2006)
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