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Category CDBセミナー
Date and Time 2017-01-10 15:00 - 16:00
Venue Seminar Room A7F
Speaker Kristin Gunsalus
Affiliation York University
Title [QBiC CDB Joint Seminar] Functional Genomics, Chemical Genetics, and Natural Products Discovery
Poster click here to download(PDF)
Host Shuichi Onami
Summary Genetic interaction mapping and high-content phenotypic analysis provide a powerful
means to characterize biological functions of chemical and genetic perturbations in living
systems. We have developed mammalian cell-based assays and whole organism screens
using the animal model C. elegans that we are using in a variety of applications. In our
previous work we have performed genome-wide RNAi screens coupled with high-content
profiling to identify genes that interfere with germline or embryonic development and
analyze their cell biological phenotypes. We are now extending this work to pairwise
genetic interactions in C. elegans to study how essential and non-essential genes work
together to promote proper animal development and metabolic homeostasis. We have
also developed panels of mammalian cell lines that allow us to monitor changes in
multiple cellular compartments, the cytoskeleton, and signaling pathways using highcontent
imaging. We are using both of these systems to identify novel bioactive
molecules isolated from unique natural habitats in coastal marine areas. The combination
of genetic and chemical perturbations with high-throughput and high-content phenotypic
screens offers an opportunity to rapidly identify modes of action for unknown chemical
compounds. We are applying these approaches to identify and characterize bioactive
natural products as candidates for novel biological probes and potential therapeutic
applications.
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